Dr Ligia PrezottoLigia Prezotto Cryostat

Dr Jennifer ThorsonJennifer Thorson Blue

The Nutritional & Reproductive Physiology Laboratory was established by Drs Ligia Prezotto and Jennifer Thorson in 2015 to evaluate the effects of diet on molecular mechanisms within the gastrointestinal and reproductive systems of livestock. Established techniques within the laboratory include immunohistochemistry, colorimetric and fluorescent microscopy, neuroanatomical microdissection, enzyme-linked immunosorbent assays, and doppler ultrasonography.

 

Immunohistochemistry: The team recently completed a short sabbatical at the Institut national de la santé et de la recherche médicale (INSERM) with Vincent Prevot in Lille, France. The purpose of the visit was to learn a technique used to stain the structures of the blood-brain barrier that has been applied in species such as mice and rats. They will now implement this technique in the Nutritional & Reproductive Physiology Laboratory using bovine as a model.

Mouse Hypothalamus

 

Doppler ultrasounography: Series of five images from the same fetus taken approximately 45 days after insemination. Images transect the fetus along the sagittal plane. The head is positioned on the right side of all images with the open mouth pointing towards 10 o'clock. Coloration (blue and red) indicate blood flow within the fetal heart (Images A-E), brain (Images B-E), and vasculature between (Image E).

Doppler Ultrasound

 

Students

Name University Title Time in Service
Cole McCann University of Montana - Western Undergraduate Research Assistant May 2016 - June 2016
Riley Dennis Montana State University - Northern Undergraduate Research Assistant September 2016 - Present
José Vitor Pizol University of São Paulo - Pirassununga International Intern January 2017 - August 2017
Marley Manoukian
Montana State University - Bozeman Montana Agricultural Experiment Station Summer Intern June 2017 - August 2017