Effect of Salicylate on Wheat Resistance to Orange Wheat Blossom Midge
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Objective:
To evaluate the efficacy of Salicylate for the control of OWBM in susceptible and resistant spring wheat cultivars.
Materials and Methods:
Spring wheat varieties containing the Sm1 gene for resistance to the orange wheat blossom midge are available to aid in their control. However, some damage is incurred prior to the synthesis of the active compound. This study was designed to determine if treatment with salicylate prior to larval feeding could upregulate the Sm1 gene and shorten the lag phase. This study was established as a split plot design with three replications. Egan, a cultivar with resistance to the OWBM, and McNeal, a non-resistant cultivar were the whole plot treatments. Salicylate was applied to both varieties at three rates and at three wheat growth stages. Salicylate was applied at 0, 21, and 42 g ai/A when wheat was at the late boot stage, 50% headed, and 50% flowering growth stages.
Results:
The study site experienced severe drought and low midge pressure. As a result, any treatment effects that might have been present were largely masked by these environmental conditions. However, a few treatment effects were detected, and were largely associated with the differences between the two spring wheat varieties. Spring wheat yields averaged 23.6 bu/A, with McNeal producing slightly higher yields then Egan (24.0 and 23.2 bu/A, respectively). Egan had greater protein and higher falling numbers, but McNeal had higher test weight and greater thousand kernel weight. There were trends in the data which indicated a slight reduction in height, yield and test weight as rates of salicylate increased (Pr>F 0.4162, 0.1139, and 0.0519, respectively), while protein tended to increase (Pr>F=0.1148). However, the timing of salicylate treatments had no impact on any of the variables measured.
Summary:
Record breaking drought and low midge populations prevented an accurate assessment of salicylate for improving crop resistance against the wheat midge.
Table 1. Materials and Methods - Sm1 Salicylate |
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Seeding Date: | 5/6/2015 | Harvest Date: | 8/12/2015 |
Julian Date: | 125 | Julian Date: | 224 |
Seeding Rate: | 80 lb/A | Soil Type: | Somers Silty Clay Loam |
Previous Crop: | Spring Wheat | Fertilizer (PP): | 23-55-30-22 |
Tillage: | Conventional | Fertilizer (TD): | 1.4Zn-200N |
Irrigation: | None | Pesticide: | None |
PP: pre-plant, TD: top dress |
Table 2. Main effect of application timing |
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HT | LOD | YLD¹ | PRO² | TWT¹ | TKW¹ | FN | OWBM | |
in | % | bu/A | % | lb/bu | g | sec | no/ spk | |
Late Boot | 21.1 | 0.0 | 23.5 | 17.4 | 53.5 | 24.4 | 521.2 | 0.0 |
50% Heading | 31.9 | 0.0 | 23.5 | 17.6 | 53.6 | 24.8 | 526.7 | 0.0 |
50% Flowering | 20.8 | 0.0 | 23.9 | 17.3 | 53.9 | 25.1 | 526.5 | 0.0 |
LSD | ns | ns | ns | ns | ns | ns | ns | ns |
Pr>F | 0.4284 | 1.0000 | 0.9314 | 0.7382 | 0.5950 | 0.4983 | 0.6398 | 0.7849 |
HT; height, LOD: lodging, YLD: yield, PRO: protein, TWT: test weight, TKW: thousand kernel weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |
Table 3. Main effect of treatment rate |
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HT | LOD | YLD¹ | PRO² | TWT¹ | TKW¹ | FN | OWBM | |
in | % | bu/A | % | lb/bu | g | sec | no/ spk | |
Untreated Check | 31.5 | 0.0 | 24.5 | 17.1 | 54.0 | 25.0 | 524.9 | 0.0 |
Salicylic acid 21 g ai/A | 21.3 | 0.0 | 23.6 | 17.5 | 53.6 | 24.5 | 526.1 | 0.0 |
Salicylic acid 42 g ai/A | 21.0 | 0.0 | 22.7 | 17.7 | 53.5 | 24.8 | 523.4 | 0.0 |
LSD | ns | ns | ns | ns | 0.4 | ns | ns | ns |
Pr>F | 0.4162 | 1.0000 | 0.1139 | 0.1148 | 0.0519 | 0.4177 | 0.9195 | 0.6380 |
HT; height, LOD: lodging, YLD: yield, PRO: protein, TWT: test weight, TKW: thousand kernel weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |
Table 4. Main effect of variety |
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HT | LOD | YLD¹ | PRO² | TWT¹ | TKW¹ | FN | OWBM | |
in | % | bu/A | % | lb/bu | g | sec | no/ spk | |
Egan | 20.7 | 0.0 | 23.2 | 18.0 | 53.0 | 23.7 | 533.1 | 0.0 |
McNeal | 28.6 | 0.0 | 24.0 | 16.8 | 54.4 | 25.8 | 516.6 | 0.0 |
LSD | ns | ns | 0.7 | 0.4 | 0.3 | 0.6 | 10.8 | ns |
Pr>F | 0.2797 | 1.0000 | 0.0205 | 0.0001 | 0.0001 | 0.0001 | 0.0048 | 0.1510 |
HT; height, LOD: lodging, YLD: yield, PRO: protein, TWT: test weight, TKW: thousand kernel weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |
Table 5. Effect of application timing and rate of treatment |
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HT | LOD | YLD¹ | PRO² | TWT¹ | TKW¹ | FN | OWBM | |
in | % | bu/A | % | lb/bu | g | sec | no/ spk | |
Check | ||||||||
Late Boot | 21.2 | 0.0 | 25.3 | 17.0 | 53.8 | 24.8 | 523.2 | 0.0 |
50% Heading | 52.8 | 0.0 | 24.6 | 17.1 | 54.1 | 25.3 | 523.1 | 0.0 |
50% Flowering | 20.5 | 0.0 | 23.8 | 17.1 | 54.0 | 25.0 | 528.3 | 0.0 |
Salicylic acid 21 g ai/A | ||||||||
Late Boot | 21.3 | 0.0 | 23.3 | 17.3 | 53.6 | 24.3 | 517.9 | 0.0 |
50% Heading | 21.5 | 0.0 | 24.0 | 17.6 | 53.4 | 24.3 | 530.6 | 0.1 |
50% Flowering | 21.2 | 0.0 | 23.7 | 17.5 | 53.8 | 24.8 | 529.8 | 0.1 |
Salicylic acid 42 g ai/A | ||||||||
Late Boot | 20.8 | 0.0 | 22.0 | 17.9 | 53.1 | 23.9 | 522.3 | 0.0 |
50% Heading | 21.3 | 0.0 | 21.9 | 17.9 | 53.4 | 24.8 | 526.5 | 0.0 |
50% Flowering | 20.8 | 0.0 | 24.1 | 17.2 | 54.0 | 25.6 | 521.5 | 0.1 |
LSD | ns | ns | ns | ns | ns | ns | ns | ns |
Pr>F | 0.4536 | 1.0000 | 0.4226 | 0.6385 | 0.4195 | 0.5898 | 0.8834 | 0.3361 |
HT: height, LOD: lodging, YLD: yield, PRO: protein, TWT: test weight, TKW: thousand kernal weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |
Table 6. Effect of application timing and variety |
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HT | LOD | YLD¹ |
PRO² | TWT¹ | TKW¹ | FN | OWBM | |
in | % | bu/A | % | lb/bu | g | sec | no/ spk | |
Egan | ||||||||
Late Boot | 20.4 | 0.0 | 23.3 | 17.8 | 53.0 | 23.5 | 526.4 | 0.0 |
50% Heading | 21.1 | 0.0 | 22.8 | 18.1 | 52.8 | 23.4 | 535.6 | 0.0 |
50% Flowering | 20.4 | 0.0 | 23.5 | 18.0 | 53.1 | 24.0 | 537.1 | 0.0 |
McNeal | ||||||||
Late Boot | 21.8 | 0.0 | 23.7 | 17.0 | 54.0 | 25.2 | 515.9 | 0.0 |
50% Heading | 42.7 | 0.0 | 24.1 | 17.0 | 54.4 | 26.1 | 517.9 | 0.1 |
50% Flowering | 21.2 | 0.0 | 24.2 | 16.5 | 54.7 | 26.2 | 515.9 | 0.1 |
LSD | ns | ns | ns | ns | ns | ns | ns | ns |
Pr>F | 0.4118 | 1.0000 | 0.5437 | 0.3703 | 0.1814 | 0.4151 | 0.6917 | 0.2130 |
HT: height, LOD: lodging, YLD: yield, PRO: protein, TWT: test weight, TKW: thousand kernal weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |
Table 7. Effect of treatment rate and variety |
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HT | LOD | YLD¹ | PRO² | TWT¹ | TKW¹ | FN | OWBM | |
in | % | bu/A | % | lb/bu | g | sec | no/ spk | |
Egan | ||||||||
Untreated Check | 20.7 | 0.0 | 24.5 | 17.5 | 53.5 | 24.1 | 531.4 | 0.0 |
Salicylic acid 21 g ai/A | 21.0 | 0.0 | 23.2 | 18.1 | 52.9 | 23.3 | 536.4 | 0.0 |
Salicylic acid 42 g ai/A | 20.3 | 0.0 | 21.9 | 18.3 | 52.7 | 23.6 | 531.4 | 0.0 |
McNeal | ||||||||
Untreated Check | 42.3 | 0.0 | 24.6 | 16.7 | 54.5 | 26.0 | 518.3 | 0.0 |
Salicylic acid 21 g ai/A | 21.7 | 0.0 | 24.1 | 16.8 | 54.4 | 25.6 | 515.9 | 0.1 |
Salicylic acid 42 g ai/A | 21.7 | 0.0 | 23.4 | 17.0 | 54.3 | 25.9 | 515.5 | 0.1 |
LSD | ns | ns | ns | ns | ns | ns | ns | ns |
Pr>F | 0.4060 | 1.0000 | 0.2903 | 0.4796 | 0.2222 | 0.8449 | 0.8413 | 0.5795 |
HT: height, LOD: lodging, YLD: yield, PRO: protein, TWT: test weight, TKW: thousand kernal weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |
Table 8. Effect of application timing, treatment rate and variety |
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Height (in) | Lodging (%) | Yield¹ (bu/A) | Protein² (%) | TWT¹ (lb/bu) | TKW¹ (g) | FN (sec) | OWBM (no/spk) | |||||||||
Egan | McNeal | Egan | McNeal | Egan | McNeal | Egan | McNeal | Egan | McNeal | Egan | McNeal | Egan | McNeal | Egan | McNeal | |
Check | ||||||||||||||||
Late Boot | 20.7 | 21.7 | 0.0 | 0.0 | 25.7 | 24.9 | 16.9 | 17.1 | 53.7 | 53.8 | 24.3 | 25.4 | 524.1 | 522.3 | 0.1 | 0.0 |
50% Heading | 21.0 | 84.7 | 0.0 | 0.0 | 23.8 | 25.3 | 17.8 | 16.5 | 53.3 | 54.8 | 23.9 | 26.7 | 534.6 | 511.6 | 0.0 | 0.1 |
50% Flowering | 20.3 | 20.7 | 0.0 | 0.0 | 23.9 | 23.7 | 17.7 | 16.5 | 53.3 | 54.8 | 24.0 | 26.0 | 535.5 | 521.0 | 0.0 | 0.0 |
Salicylic acid 21 g ai/A | ||||||||||||||||
Late Boot | 20.7 | 22.0 | 0.0 | 0.0 | 23.3 | 23.3 | 17.8 | 16.7 | 53.0 | 54.2 | 23.4 | 25.2 | 536.6 | 499.2 | 0.0 | 0.0 |
50% Heading | 21.3 | 21.7 | 0.0 | 0.0 | 23.5 | 24.5 | 18.1 | 17.1 | 52.6 | 54.2 | 22.7 | 25.8 | 531.5 | 529.8 | 0.1 | 0.1 |
50% Flowering | 21.0 | 21.3 | 0.0 | 0.0 | 22.9 | 24.4 | 18.4 | 16.6 | 52.9 | 54.7 | 23.7 | 25.8 | 541.0 | 518.7 | 0.0 | 0.1 |
Salicylic acid 42 g ai/A | ||||||||||||||||
Late Boot | 20.0 | 21.7 | 0.0 | 0.0 | 20.9 | 23.1 | 18.6 | 17.2 | 52.2 | 54.0 | 22.9 | 25.0 | 518.5 | 526.2 | 0.0 | 0.0 |
50% Heading | 21.0 | 21.7 | 0.0 | 0.0 | 21.2 | 22.5 | 18.5 | 17.3 | 52.6 | 54.1 | 23.7 | 25.9 | 540.7 | 512.3 | 0.0 | 0.0 |
50% Flowering | 20.0 | 21.7 | 0.0 | 0.0 | 23.8 | 24.4 | 17.9 | 16.5 | 53.2 | 54.7 | 24.4 | 26.8 | 534.9 | 508.1 | 0.0 | 0.2 |
LSD | ns | ns | ns | ns | ns | ns | ns | ns | ||||||||
Pr>F | 0.4324 | 1.0000 | 0.3365 | 0.5002 | 0.3449 | 0.8856 | 0.2147 | 0.6929 | ||||||||
TWT: test weight, TKW: thousand kernal weight, FN: falling number, OWBM: orange wheat blossom midge, ns: nonsignificant | ||||||||||||||||
¹adjusted to 13% moisture, ²adjusted to 12% |